ASCP Histotechnician (ASCP-HT) Practice Tests & Test Prep by Exam Edge

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ASCP Histotechnician Sample Test

1 of 5

The most effective penetration of the fixative is at how many mms for osmium?

Correct Answer:

0.5mm.
the most effective penetration of the fixative, when using osmium, is at 0.5 mm.

fixation is a critical process in preparing tissue samples for microscopic examination. the primary goal of fixation is to preserve tissues in as close to their natural state as possible by halting biological processes and preventing decay. this is achieved through two main steps: stopping the tissue's normal biological functions and preserving the tissue's structure and molecular composition.

there are three important parameters to consider during the fixation process to ensure optimal preservation of the tissue's microanatomy and molecular integrity. firstly, the time between the cessation of life functions in the tissue and the start of the fixation process should be minimized to prevent autolysis and other degradative processes. secondly, the size of the tissue sample should be kept as small as feasible. this ensures that the fixative can penetrate the tissue evenly and rapidly, which is crucial for effective fixation. lastly, it is important to minimize any gross deformation of the tissue during handling and processing to maintain the tissue's structural integrity.

specifically for osmium tetroxide (oso4), a common fixative used in electron microscopy for its ability to fix lipids and provide excellent contrast, the effective penetration depth is particularly limited. osmium tetroxide penetrates tissue at an effective depth of only about 0.5 mm. this shallow penetration depth necessitates the preparation of very small tissue samples to ensure that the fixative can effectively reach all parts of the sample. larger samples may not be adequately fixed in their interior, potentially leading to artifacts or degradation of tissue structure and composition in these areas.

therefore, when preparing tissue samples for fixation with osmium tetroxide, it is critical to slice the tissue into thin sections, ideally no thicker than 0.5 mm. this practice ensures that the fixative can fully penetrate the sample, achieving thorough fixation, which is essential for high-quality microscopic analysis.

ASCP Histotechnician (ASCP-HT) Shortcuts

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Exam Edge practice tests are a valuable tool for helping you prepare for the American Society for Clinical Pathology (ASCP-HT) certification exam. The practice tests provide a realistic simulation of the actual exam, allowing you to become familiar with the format, style, and types of questions you will encounter on the actual test. Certification exams don't just measure what you know -- they are also a test of how well you perform under pressure. The right type of test preparation helps you familiarize yourself not only with the material you're being tested on, but also the format of the test, so you feel less anxiety on test day.

ASCP Histotechnician Aliases Test Name

Here is a list of alternative names used for this exam.

ASCP Histotechnician
ASCP Histotechnician test
ASCP Histotechnician Certification Test
ASCP
ASCP ASCP-HT
ASCP-HT test
ASCP Histotechnician (ASCP-HT)
Histotechnician certification