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ASCP Histotechnologist International (ASCP-HTLI) Practice Tests & Test Prep by Exam Edge - Free Test


Our free ASCP Histotechnologist International (ASCP-HTLI) Practice Test was created by experienced educators who designed them to align with the official American Society for Clinical Pathology content guidelines. They were built to accurately mirror the real exam's structure, coverage of topics, difficulty, and types of questions.

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ASCP Histotechnologist International - Free Test Sample Questions

Once the paraffin wax has cooled, what is done next?





Correct Answer:
tissue blocks are sectioned.


once the paraffin wax has cooled, the embedded tissue blocks are ready for the next step in the histological preparation process, which is sectioning. sectioning involves cutting the tissue blocks into very thin slices, which can be as thin as a few micrometers, typically using a device known as a microtome. this step is crucial as it allows for the detailed examination of the tissue's microstructure under a microscope.

the microtome holds the tissue block in place while a sharp blade moves across it to shave off thin sections. these sections are collected on slides to be stained and later examined. the orientation of the sectioning can vary depending on the specific structural details that need to be observed. while horizontal and vertical orientations are common, the choice largely depends on the histological structure that is being targeted for study.

the quality of the sectioning depends significantly on the hardness of the paraffin wax, the precision of the microtome, and the skill of the technician. properly sectioned tissues allow for optimal staining and detailed microscopic analysis, which is critical for accurate diagnosis and research findings. therefore, sectioning is a skillful art that combines the science of histology with meticulous handling and processing of tissue samples.